Western blot analysis of lysates from mouse testis, using TSSK4 Rabbit pAb (STJ110171) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TSSK4 antibody (79-328) (STJ110171)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110171
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General Information

Short DescriptionRabbit polyclonal TSSK4 (79-328) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTSSK4
Gene ID283629
Uniprot IDTSSK4_HUMAN
Immunogen Region79-328
Immunogen SequenceVLRHKYLINFYRAIESTSRV YIILELAQGGDVLEWIQRYG ACSEPLAGKWFSQLTLGIAY LHSKSIVHRDLKLENLLLDK WENVKISDFGFAKMVPSNQP VGCSPSYRQVNCFSHLSQTY CGSFAYACPEILRGLPYNPF LSDTWSMGVILYTLVVAHLP FDDTNLKKLLRETQKEVTFP ANHTISQECKNLILQMLRQA TKRATILDIIKDSWVLKFQP EQPTHEIRLLEAMCQLHNT
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 79-328 of human TSSK4 (NP_777604.2).

Additional Info

Tissue Specificity Expressed only in the testis.
Post Translational Modifications Activated by autophosphorylation on Thr-197. ODF2 potentiates the autophosphorylation activity of TSSK4 at Thr-197. Ubiquitinated.HSP90 activity negatively regulates ubiquitination and degradation.
Function Serine/threonine kinase which is involved in male germ cell development and in mature sperm function. May be involved in the Cre/Creb signaling pathway. Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells. Phosphorylates CREM on 'Ser-116' in vitro. Phosphorylates ODF2 on 'Ser-95'.
Protein Name Testis-Specific Serine/Threonine-Protein Kinase 4
Tsk-4
Tssk-4
Testis-Specific Kinase 4
Serine/Threonine-Protein Kinase 22e
Database Links
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cell Projection
Cilium
Flagellum
In Spermatozoa
Present In The Sperm Head And In The Flagellum
Alternative Antibody Names Anti-Testis-Specific Serine/Threonine-Protein Kinase 4 antibody
Anti-Tsk-4 antibody
Anti-Tssk-4 antibody
Anti-Testis-Specific Kinase 4 antibody
Anti-Serine/Threonine-Protein Kinase 22e antibody
Anti-TSSK4 antibody
Anti-C14orf20 antibody
Anti-STK22E antibody
Anti-TSSK5 antibody

Information sourced from Uniprot.org

Citations

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