Anti-BUB3 antibody (1-328) (STJ28619)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BUB3 (1-328) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BUB3 |
| Gene ID | 9184 |
| Uniprot ID | BUB3_HUMAN |
| Immunogen Region | 1-328 |
| Immunogen Sequence | MTGSNEFKLNQPPEDGISSV KFSPNTSQFLLVSSWDTSVR LYDVPANSMRLKYQHTGAVL DCAFYDPTHAWSGGLDHQLK MHDLNTDQENLVGTHDAPIR CVEYCPEVNVMVTGSWDQTV KLWDPRTPCNAGTFSQPEKV YTLSVSGDRLIVGTAGRRVL VWDLRNMGYVQQRRESSLKY QTRCIRAFPNKQGYVLSSIE GRVAVEYLDPSPEVQKKKYA FKCHRLKENNIEQIYPVNA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1). |
Additional Info
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex. |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Protein Name | Mitotic Checkpoint Protein Bub3 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreStarts To Localize At Kinetochores In Prometaphase I (Pro-Mi) Stage And Maintains The Localization Until The Metaphase I-Anaphase I (Mi-Ai) Transition |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Protein Bub3 antibodyAnti-BUB3 antibody |
Information sourced from Uniprot.org