Western blot analysis of lysates from mouse testis, using TSSK4 Rabbit pAb (STJ110171) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TSSK4 antibody (79-328) (STJ110171)

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STJ110171
€138.13 - €631.88
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSSK4
Gene ID: 283629
Uniprot ID: TSSK4_HUMAN
Immunogen Region: 79-328
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 79-328 of human TSSK4 (NP_777604.2).
Immunogen Sequence: VLRHKYLINFYRAIESTSRV YIILELAQGGDVLEWIQRYG ACSEPLAGKWFSQLTLGIAY LHSKSIVHRDLKLENLLLDK WENVKISDFGFAKMVPSNQP VGCSPSYRQVNCFSHLSQTY CGSFAYACPEILRGLPYNPF LSDTWSMGVILYTLVVAHLP FDDTNLKKLLRETQKEVTFP ANHTISQECKNLILQMLRQA TKRATILDIIKDSWVLKFQP EQPTHEIRLLEAMCQLHNT
Tissue Specificity Expressed only in the testis.
Post Translational Modifications Activated by autophosphorylation on Thr-197. ODF2 potentiates the autophosphorylation activity of TSSK4 at Thr-197. Ubiquitinated.HSP90 activity negatively regulates ubiquitination and degradation.
Function Serine/threonine kinase which is involved in male germ cell development and in mature sperm function. May be involved in the Cre/Creb signaling pathway. Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells. Phosphorylates CREM on 'Ser-116' in vitro. Phosphorylates ODF2 on 'Ser-95'.
Protein Name Testis-Specific Serine/Threonine-Protein Kinase 4
Tsk-4
Tssk-4
Testis-Specific Kinase 4
Serine/Threonine-Protein Kinase 22e
Database Links
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cell Projection
Cilium
Flagellum
In Spermatozoa
Present In The Sperm Head And In The Flagellum
Alternative Antibody Names Anti-Testis-Specific Serine/Threonine-Protein Kinase 4 antibody
Anti-Tsk-4 antibody
Anti-Tssk-4 antibody
Anti-Testis-Specific Kinase 4 antibody
Anti-Serine/Threonine-Protein Kinase 22e antibody
Anti-TSSK4 antibody
Anti-C14orf20 antibody
Anti-STK22E antibody
Anti-TSSK5 antibody

Information sourced from Uniprot.org

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