Anti-TRIM23 antibody (1-280) (STJ110627)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM23 (1-280) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM23 |
| Gene ID | 373 |
| Uniprot ID | TRI23_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MATLVVNKLGAGVDSGRQGS RGTAVVKVLECGVCEDVFSL QGDKVPRLLLCGHTVCHDCL TRLPLHGRAIRCPFDRQVTD LGDSGVWGLKKNFALLELLE RLQNGPIGQYGAAEESIGIS GESIIRCDEDEAHLASVYCT VCATHLCSECSQVTHSTKTL AKHRRVPLADKPHEKTMCSQ HQVHAIEFVCLEEGCQTSPL MCCVCKEYGKHQGHKHSVLE PEANQIRASILDMAHCIRT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM23 (NP_001647.1). |
Additional Info
| Function | Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes. (Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim23Adp-Ribosylation Factor Domain-Containing Protein 1Gtp-Binding Protein Ard-1Ring Finger Protein 46Ring-Type E3 Ubiquitin Transferase Trim23Tripartite Motif-Containing Protein 23 |
| Database Links | |
| Cellular Localisation | CytoplasmEndomembrane SystemGolgi Apparatus MembraneLysosome MembraneMembrane-Associated With The Golgi Complex And Lysosomal Structures |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim23 antibodyAnti-Adp-Ribosylation Factor Domain-Containing Protein 1 antibodyAnti-Gtp-Binding Protein Ard-1 antibodyAnti-Ring Finger Protein 46 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim23 antibodyAnti-Tripartite Motif-Containing Protein 23 antibodyAnti-TRIM23 antibodyAnti-ARD1 antibodyAnti-ARFD1 antibodyAnti-RNF46 antibody |
Information sourced from Uniprot.org