• Western blot analysis of extracts of various cell lines, using TRIM23 antibody (STJ110627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of NIH/3T3 cells using TRIM23 Rabbit polyclonal antibody (STJ110627) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TRIM23 rabbit polyclonal antibody (STJ110627) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TRIM23 antibody (STJ110627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TRIM23 antibody (1-280) (STJ110627)

SKU:
STJ110627

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM23 (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM23
Gene ID: 373
Uniprot ID: TRI23_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM23 (NP_001647.1).
Immunogen Sequence: MATLVVNKLGAGVDSGRQGS RGTAVVKVLECGVCEDVFSL QGDKVPRLLLCGHTVCHDCL TRLPLHGRAIRCPFDRQVTD LGDSGVWGLKKNFALLELLE RLQNGPIGQYGAAEESIGIS GESIIRCDEDEAHLASVYCT VCATHLCSECSQVTHSTKTL AKHRRVPLADKPHEKTMCSQ HQVHAIEFVCLEEGCQTSPL MCCVCKEYGKHQGHKHSVLE PEANQIRASILDMAHCIRT
Function Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes. (Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection.
Protein Name E3 Ubiquitin-Protein Ligase Trim23
Adp-Ribosylation Factor Domain-Containing Protein 1
Gtp-Binding Protein Ard-1
Ring Finger Protein 46
Ring-Type E3 Ubiquitin Transferase Trim23
Tripartite Motif-Containing Protein 23
Cellular Localisation Cytoplasm
Endomembrane System
Golgi Apparatus Membrane
Lysosome Membrane
Membrane-Associated With The Golgi Complex And Lysosomal Structures
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim23 antibody
Anti-Adp-Ribosylation Factor Domain-Containing Protein 1 antibody
Anti-Gtp-Binding Protein Ard-1 antibody
Anti-Ring Finger Protein 46 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim23 antibody
Anti-Tripartite Motif-Containing Protein 23 antibody
Anti-TRIM23 antibody
Anti-ARD1 antibody
Anti-ARFD1 antibody
Anti-RNF46 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance