• Western blot analysis of extracts of various cell lines, using TIMM23 antibody (STJ116323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of A549 cells using TIMM23 rabbit polyclonal antibody (STJ116323) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TIMM23 rabbit polyclonal antibody (STJ116323) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TIMM23 antibody (7-209) (STJ116323)

SKU:
STJ116323

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMM23 (7-209) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM23
Gene ID: 100287932
Uniprot ID: TIM23_HUMAN
Immunogen Region: 7-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 7-209 of human TIMM23 (NP_006318.1).
Immunogen Sequence: SGNKTTGGLAGFFGAGGAGY SHADLAGVPLTGMNPLSPYL NVDPRYLVQDTDEFILPTGA NKTRGR
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim23
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim23 antibody
Anti-TIMM23 antibody
Anti-TIM23 antibody

Information sourced from Uniprot.org

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