Western blot analysis of various lysates using TIMM23 Rabbit pAb (STJ116323) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-TIMM23 antibody (7-209) (STJ116323)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ116323
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General Information

Short DescriptionRabbit polyclonal TIMM23 (7-209) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTIMM23
Gene ID100287932
Uniprot IDTIM23_HUMAN
Immunogen Region7-209
Immunogen SequenceSGNKTTGGLAGFFGAGGAGY SHADLAGVPLTGMNPLSPYL NVDPRYLVQDTDEFILPTGA NKTRGR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 7-72 of human TIMM23 (NP_006318.1).

Additional Info

Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has a role in the activation of stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation and facilitating its accumulation at the outer mitochondrial membrane in response to depolarization.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim23
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim23 antibody
Anti-TIMM23 antibody
Anti-TIM23 antibody

Information sourced from Uniprot.org

Citations

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