| Short Description : | Rabbit polyclonal THUMPD3 (1-250) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | THUMPD3 |
| Gene ID: | 25917 |
| Uniprot ID: | THUM3_HUMAN |
| Immunogen Region: | 1-250 |
| Immunogen Sequence: | MCDIEEATNQLLDVNLHENQ KSVQVTESDLGSESELLVTI GATVPTGFEQTAADEVREKL GSSCKISRDRGKIYFVISVE SLAQVHCLRSVDNLFVVVQE FQDYQFKQTKEEVLKDFEDL AGKLPWSNPLKVWKINASFK KKKAKRKKINQNSSKEKINN GQEVKIDQRNVKKEFTSHAL DSHILDYYENPAIKEDVSTL IGDDLASCKDETDESSKEET EPQVLKFRVTCNRAGEKHC |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human THUMPD3 (NP_056268.2). |
| Function | Catalytic subunit of the THUMPD3-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 6 (m2G6) in tRNAs. This is one of the major tRNA (guanine-N(2))-methyltransferases. Also catalyzes the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 7 of tRNA(Trp). |
| Protein Name | TrnaGuanine(6-N(2-Methyltransferase Thump3Thump Domain-Containing Protein 3Trna(TrpGuanine(7-N(2-Methyltransferase Thump3 |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Guanine(6-N(2-Methyltransferase Thump3 antibodyAnti-Thump Domain-Containing Protein 3 antibodyAnti-Trna(Trp antibodyAnti-Guanine(7-N(2-Methyltransferase Thump3 antibodyAnti-THUMPD3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/88716/404925/STJ110441_1__17740.1744890652.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
