• Immunohistochemistry analysis of paraffin-embedded rat ovary using THUMPD3 Rabbit polyclonal antibody (STJ112442) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using THUMPD3 Rabbit polyclonal antibody (STJ112442) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using THUMPD3 Rabbit polyclonal antibody (STJ112442) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using THUMPD3 antibody (STJ112442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-THUMPD3 antibody (1-250) (STJ112442)

SKU:
STJ112442

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-THUMPD3 (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: THUMPD3
Gene ID: 25917
Uniprot ID: THUM3_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human THUMPD3 (NP_056268.2).
Immunogen Sequence: MCDIEEATNQLLDVNLHENQ KSVQVTESDLGSESELLVTI GATVPTGFEQTAADEVREKL GSSCKISRDRGKIYFVISVE SLAQVHCLRSVDNLFVVVQE FQDYQFKQTKEEVLKDFEDL AGKLPWSNPLKVWKINASFK KKKAKRKKINQNSSKEKINN GQEVKIDQRNVKKEFTSHAL DSHILDYYENPAIKEDVSTL IGDDLASCKDETDESSKEET EPQVLKFRVTCNRAGEKHC
Function Methyltransferase which catalyzes the formation of N(2)-methylguanosine at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs. Also catalyzes the formation of N(2)-methylguanosine at position 7 of tRNA(Trp). Requires the methyltransferase adapter protein TRM112 for tRNA methyltransferase activity.
Protein Name Trna
Guanine(6-N2-Methyltransferase Thump3
Thump Domain-Containing Protein 3
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Trna antibody
Anti-Guanine(6-N2-Methyltransferase Thump3 antibody
Anti-Thump Domain-Containing Protein 3 antibody
Anti-THUMPD3 antibody

Information sourced from Uniprot.org

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