• Western blot analysis of extracts of various cell lines, using TEFM antibody (STJ110809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal immunofluorescence analysis of U2OS cells using TEFM Rabbit polyclonal antibody (STJ110809) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat lung using TEFM antibody (STJ110809) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of TEFM in paraffin-embedded mouse spleen using TEFM Rabbit polyclonal antibody (STJ110809) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using TEFM antibody (STJ110809) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of TEFM in paraffin-embedded human colon carcinoma using TEFM Rabbit polyclonal antibody (STJ110809) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody (STJ110809) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of TEFM in paraffin-embedded rat lung using TEFM Rabbit polyclonal antibody (STJ110809) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal immunofluorescence analysis of U2OS cells using TEFM Polyclonal Antibody (STJ110809) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from A549 cells, using TEFM Rabbit polyclonal antibody (STJ110809) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-TEFM antibody (36-210) (STJ110809)

SKU:
STJ110809

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TEFM (36-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TEFM
Gene ID: 79736
Uniprot ID: TEFM_HUMAN
Immunogen Region: 36-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3).
Immunogen Sequence: KSTTPKKITPNVTFCDENAK EPENALDKLFSSEQQASILH VLNTASTKELEAFRLLRGRR SINIVEHRENFGPFQNLESL MNVPLFKYKSTVQVCNSILC PKTGREKRKSPENRFLRKLL KPDIERERLKAVNSIISIVF GTRRIAWAHLDRKLTVLDWQ QSDRWSLMRGIYSSS
Function Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery.
Protein Name Transcription Elongation Factor - Mitochondrial
Cellular Localisation Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Transcription Elongation Factor - Mitochondrial antibody
Anti-TEFM antibody
Anti-C17orf42 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance