Western blot analysis of lysates from A549 cells, using TEFM Rabbit pAb (STJ110809) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-TEFM antibody (36-210) (STJ110809)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110809
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General Information

Short DescriptionRabbit polyclonal TEFM (36-210) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTEFM
Gene ID79736
Uniprot IDTEFM_HUMAN
Immunogen Region36-210
Immunogen SequenceKSTTPKKITPNVTFCDENAK EPENALDKLFSSEQQASILH VLNTASTKELEAFRLLRGRR SINIVEHRENFGPFQNLESL MNVPLFKYKSTVQVCNSILC PKTGREKRKSPENRFLRKLL KPDIERERLKAVNSIISIVF GTRRIAWAHLDRKLTVLDWQ QSDRWSLMRGIYSSS
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3).

Additional Info

Function Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery.
Protein Name Transcription Elongation Factor - Mitochondrial
Database Links
Cellular Localisation Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Transcription Elongation Factor - Mitochondrial antibody
Anti-TEFM antibody
Anti-C17orf42 antibody

Information sourced from Uniprot.org

Citations

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