| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TEFM |
| Gene ID: | 79736 |
| Uniprot ID: | TEFM_HUMAN |
| Immunogen Region: | 36-210 |
| Immunogen Sequence: | KSTTPKKITPNVTFCDENAK EPENALDKLFSSEQQASILH VLNTASTKELEAFRLLRGRR SINIVEHRENFGPFQNLESL MNVPLFKYKSTVQVCNSILC PKTGREKRKSPENRFLRKLL KPDIERERLKAVNSIISIVF GTRRIAWAHLDRKLTVLDWQ QSDRWSLMRGIYSSS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3). |
| Function | Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery. |
| Protein Name | Transcription Elongation Factor - Mitochondrial |
| Database Links | |
| Cellular Localisation | Mitochondrion MatrixMitochondrion Nucleoid |
| Alternative Antibody Names | Anti-Transcription Elongation Factor - Mitochondrial antibodyAnti-TEFM antibodyAnti-C17orf42 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/91703/408543/STJ111285_1__83305.1744894851.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
