• Immunohistochemistry analysis of TBCB in paraffin-embedded mouse kidney using TBCB Rabbit polyclonal antibody (STJ115213) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TBCB in paraffin-embedded human placenta using TBCB Rabbit polyclonal antibody (STJ115213) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TBCB in paraffin-embedded rat brain using TBCB Rabbit polyclonal antibody (STJ115213) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TBCB Rabbit polyclonal antibody (STJ115213) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TBCB antibody (1-244) (STJ115213)

SKU:
STJ115213

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TBCB (1-244) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBCB
Gene ID: 1155
Uniprot ID: TBCB_HUMAN
Immunogen Region: 1-244
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human TBCB (NP_001272.2).
Immunogen Sequence: MEVTGVSAPTVTVFISSSLN TFRSEKRYSRSLTIAEFKCK LELLVGSPASCMELELYGVD DKFYSKLDQEDALLGSYPVD DGCRIHVIDHSGARLGEYED VSRVEKYTISQEAYDQRQDT VRSFLKRSKLGRYNEEERAQ QEAEAAQRLAEEKAQASSIP VGSRCEVRAAGQSPRRGTVM YVGLTDFKPGYWIGVRYDEP LGKNDGSVNGKRYFECQAKY GAFVKPAVVTVGDFPEEDY
Tissue Specificity Found in most tissues.
Post Translational Modifications Phosphorylation by PAK1 is required for normal function. Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. (Microbial infection) Glycosylated residues by S.typhimurium protein Ssek1: arginine GlcNAcylation promotes microtubule stability.
Function Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
Protein Name Tubulin-Folding Cofactor B
Cytoskeleton-Associated Protein 1
Cytoskeleton-Associated Protein Ckapi
Tubulin-Specific Chaperone B
Database Links Reactome: R-HSA-389977
Cellular Localisation Cytoplasm
Cytoskeleton
Colocalizes With Microtubules
In Differentiated Neurons
Located In The Cytoplasm
In Differentiating Neurons
Accumulates At The Growth Cone
Alternative Antibody Names Anti-Tubulin-Folding Cofactor B antibody
Anti-Cytoskeleton-Associated Protein 1 antibody
Anti-Cytoskeleton-Associated Protein Ckapi antibody
Anti-Tubulin-Specific Chaperone B antibody
Anti-TBCB antibody
Anti-CG22 antibody
Anti-CKAP1 antibody

Information sourced from Uniprot.org

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