Anti-SUB1 antibody (1-127) (STJ29150)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SUB1 (1-127) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SUB1 |
| Gene ID | 10923 |
| Uniprot ID | TCP4_HUMAN |
| Immunogen Region | 1-127 |
| Immunogen Sequence | MPKSKELVSSSSSGSDSDSE VDKKLKRKKQVAPEKPVKKQ KTGETSRALSSSKQSSSSRD DNMFQIGKMRYVSVRDFKGK VLIDIREYWMDPEGEMKPGR KGISLNPEQWSQLKEQISDI DDAVRKL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3). |
Additional Info
| Post Translational Modifications | Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. |
| Function | General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). |
| Protein Name | Activated Rna Polymerase Ii Transcriptional Coactivator P15Positive Cofactor 4Pc4Sub1 HomologP14 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Activated Rna Polymerase Ii Transcriptional Coactivator P15 antibodyAnti-Positive Cofactor 4 antibodyAnti-Pc4 antibodyAnti-Sub1 Homolog antibodyAnti-P14 antibodyAnti-SUB1 antibodyAnti-PC4 antibodyAnti-RPO2TC1 antibody |
Information sourced from Uniprot.org