| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SUB1 |
| Gene ID: | 10923 |
| Uniprot ID: | TCP4_HUMAN |
| Immunogen Region: | 1-127 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3). |
| Immunogen Sequence: | MPKSKELVSSSSSGSDSDSE VDKKLKRKKQVAPEKPVKKQ KTGETSRALSSSKQSSSSRD DNMFQIGKMRYVSVRDFKGK VLIDIREYWMDPEGEMKPGR KGISLNPEQWSQLKEQISDI DDAVRKL |
| Post Translational Modifications | Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. |
| Function | General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). |
| Protein Name | Activated Rna Polymerase Ii Transcriptional Coactivator P15Positive Cofactor 4Pc4Sub1 HomologP14 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Activated Rna Polymerase Ii Transcriptional Coactivator P15 antibodyAnti-Positive Cofactor 4 antibodyAnti-Pc4 antibodyAnti-Sub1 Homolog antibodyAnti-P14 antibodyAnti-SUB1 antibodyAnti-PC4 antibodyAnti-RPO2TC1 antibody |
Information sourced from Uniprot.org