Western blot analysis of various lysates using STX7 Rabbit pAb (STJ110360) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-STX7 antibody (1-170) (STJ110360)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110360
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General Information

Short DescriptionRabbit polyclonal STX7 (1-170) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSTX7
Gene ID8417
Uniprot IDSTX7_HUMAN
Immunogen Region1-170
Immunogen SequenceMSYTPGVGGDPAQLAQRISS NIQKITQCSVEIQRTLNQLG TPQDSPELRQQLQQKQQYTN QLAKETDKYIKEFGSLPTTP SEQRQRKIQKDRLVAEFTTS LTNFQKVQRQAAEREKEFVA RVRASSRVSGSFPEDSSKER NLVSWESQTQPQVQVQDEEI TEDDLRLIHE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human STX7 (NP_003560.2).

Additional Info

Tissue Specificity Highest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
Function May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Protein Name Syntaxin-7
Database Links
Cellular Localisation Early Endosome Membrane
Single-Pass Type Iv Membrane Protein
Alternative Antibody Names Anti-Syntaxin-7 antibody
Anti-STX7 antibody

Information sourced from Uniprot.org

Citations

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