• Western blot analysis of extracts of various cell lines, using STX7 antibody (STJ110360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using STX7 rabbit polyclonal antibody (STJ110360) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using STX7 rabbit polyclonal antibody (STJ110360) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using STX7 rabbit polyclonal antibody (STJ110360) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using STX7 rabbit polyclonal antibody (STJ110360) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-STX7 antibody (1-170) (STJ110360)

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STJ110360

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STX7 (1-170) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX7
Gene ID: 8417
Uniprot ID: STX7_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human STX7 (NP_003560.2).
Immunogen Sequence: MSYTPGVGGDPAQLAQRISS NIQKITQCSVEIQRTLNQLG TPQDSPELRQQLQQKQQYTN QLAKETDKYIKEFGSLPTTP SEQRQRKIQKDRLVAEFTTS LTNFQKVQRQAAEREKEFVA RVRASSRVSGSFPEDSSKER NLVSWESQTQPQVQVQDEEI TEDDLRLIHE
Tissue Specificity Highest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
Function May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Protein Name Syntaxin-7
Cellular Localisation Early Endosome Membrane
Single-Pass Type Iv Membrane Protein
Alternative Antibody Names Anti-Syntaxin-7 antibody
Anti-STX7 antibody

Information sourced from Uniprot.org

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