• Immunofluorescence analysis of MCF7 cells using SPDEF antibody (STJ116049).
  • Immunohistochemistry analysis of paraffin-embedded human placenta using SPDEF antibody (STJ116049) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of rat ovary, using SPDEF antibody (STJ116049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SPDEF antibody (1-335) (STJ116049)

SKU:
STJ116049

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPDEF (1-335) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPDEF
Gene ID: 25803
Uniprot ID: SPDEF_HUMAN
Immunogen Region: 1-335
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1).
Immunogen Sequence: MGSASPGLSSVSPSHLLLPP DTVSRTGLEKAAAGAVGLER RDWSPSPPATPEQGLSAFYL SYFDMLYPEDSSWAAKAPGA SSREEPPEEPEQCPVIDSQA PAGSLDLVPGGLTLEEHSLE QVQSMVVGEVLKDIETACKL LNITADPMDWSPSNVQKWLL WTEHQYRLPPMGKAFQELAG KELCAMSEEQFRQRSPLGGD VLHAHLDIWKSAAWMKERTS PGAIHYCASTSEESWTDSE
Tissue Specificity Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.
Function May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
Protein Name Sam Pointed Domain-Containing Ets Transcription Factor
Prostate Epithelium-Specific Ets Transcription Factor
Prostate-Specific Ets
Prostate-Derived Ets Factor
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Sam Pointed Domain-Containing Ets Transcription Factor antibody
Anti-Prostate Epithelium-Specific Ets Transcription Factor antibody
Anti-Prostate-Specific Ets antibody
Anti-Prostate-Derived Ets Factor antibody
Anti-SPDEF antibody
Anti-PDEF antibody
Anti-PSE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance