| Short Description : | Rabbit polyclonal SPDEF (1-335) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPDEF |
| Gene ID: | 25803 |
| Uniprot ID: | SPDEF_HUMAN |
| Immunogen Region: | 1-335 |
| Immunogen Sequence: | MGSASPGLSSVSPSHLLLPP DTVSRTGLEKAAAGAVGLER RDWSPSPPATPEQGLSAFYL SYFDMLYPEDSSWAAKAPGA SSREEPPEEPEQCPVIDSQA PAGSLDLVPGGLTLEEHSLE QVQSMVVGEVLKDIETACKL LNITADPMDWSPSNVQKWLL WTEHQYRLPPMGKAFQELAG KELCAMSEEQFRQRSPLGGD VLHAHLDIWKSAAWMKERTS PGAIHYCASTSEESWTDSE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1). |
| Tissue Specificity | Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells. |
| Function | May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. |
| Protein Name | Sam Pointed Domain-Containing Ets Transcription FactorProstate Epithelium-Specific Ets Transcription FactorProstate-Specific EtsProstate-Derived Ets Factor |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Sam Pointed Domain-Containing Ets Transcription Factor antibodyAnti-Prostate Epithelium-Specific Ets Transcription Factor antibodyAnti-Prostate-Specific Ets antibodyAnti-Prostate-Derived Ets Factor antibodyAnti-SPDEF antibodyAnti-PDEF antibodyAnti-PSE antibody |
Information sourced from Uniprot.org
