• Western blot analysis of various lysates using SP140 Rabbit polyclonal antibody (STJ119692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SP140 antibody (1-180) (STJ119692)

SKU:
STJ119692

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SP140 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SP140
Gene ID: 11262
Uniprot ID: SP140_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SP140 (NP_001265380).
Immunogen Sequence: MAQQGQQGQMASGDSNLNFR MVAEIQNVEGQNLQEQVCPE PIFRFFRENKVEIASAITRP FPFLMGLRDRSFISEQMYEH FQEAFRNLVPVTRVMYCVLS ELEKTFGWSHLEALFSRINL MAYPDLNEIYRSFQNVCYEH SPLQMNNVNDLEDRPRLLPY GKQENSNACHEMDDIAVPQE
Tissue Specificity High levels in spleen and peripheral blood leukocytes, much lower levels in tonsils, thymus, prostate, ovary, small intestine, and colon. Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not detected in brain, liver and muscle.
Post Translational Modifications Phosphorylation at Thr-726 promotes binding of PIN1 and subsequent isomerization of Pro-727.
Function Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. May play a role in chromatin-mediated regulation of gene expression although it does not bind to histone H3 tails.
Protein Name Nuclear Body Protein Sp140
Lymphoid-Restricted Homolog Of Sp100
Lysp100
Nuclear Autoantigen Sp-140
Speckled 140 Kda
Cellular Localisation Nucleus
Pml Body
Cytoplasm
Localized To Nuclear Structures Termed Lands
For Lysp100-Associated Nuclear Domains
Lands Are Globular
Electron-Dense Structures Most Often Found In The Nucleoplasm
But Also Found At The Nuclear Membrane And In The Cytoplasm
Suggesting That These Structures May Traffic Between The Cytoplasm And The Nucleus
Also Colocalizes With Pml In A Subset Of Pml Nuclear Bodies
Alternative Antibody Names Anti-Nuclear Body Protein Sp140 antibody
Anti-Lymphoid-Restricted Homolog Of Sp100 antibody
Anti-Lysp100 antibody
Anti-Nuclear Autoantigen Sp-140 antibody
Anti-Speckled 140 Kda antibody
Anti-SP140 antibody
Anti-LYSP100 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance