Anti-SLC25A31 antibody (131-180 aa) (STJ91607)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SLC25A31 at the amino acid range 131-180
General Information
| Short Description | Rabbit polyclonal anti-ADP/ATP translocase 4 (131-180 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC25A31 |
| Gene ID | 83447 |
| Uniprot ID | ADT4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SLC25A31 at the amino acid range 131-180 |
| Immunogen Region | 131-180 aa |
| Specificity | ANT4 Polyclonal Antibody detects endogenous levels of ANT4 protein. |
Additional Info
| Function | ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. Specifically required during spermatogenesis, probably to mediate ADP:ATP exchange in spermatocytes. Large ATP supplies from mitochondria may be critical for normal progression of spermatogenesis during early stages of meiotic prophase I, including DNA double-strand break repair and chromosomal synapsis. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A31/ANT4 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Among nucleotides, may also exchange ADP for dATP and dADP. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A31/ANT4 constitutes a pore-forming component of mPTP or regulates it. |
| Protein Name | Adp/Atp Translocase 4Adp -Atp Carrier Protein 4Adenine Nucleotide Translocator 4Ant 4Solute Carrier Family 25 Member 31Sperm Flagellar Energy Carrier Protein |
| Database Links | |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane ProteinMembraneCell ProjectionCiliumFlagellum MembraneIn Sperm Flagellum This Protein Is Located In The Fibrous SheathA Non-Mitochondrial RegionMay Localize To Non-Mitochondrial Membranes |
| Alternative Antibody Names | Anti-Adp/Atp Translocase 4 antibodyAnti-Adp -Atp Carrier Protein 4 antibodyAnti-Adenine Nucleotide Translocator 4 antibodyAnti-Ant 4 antibodyAnti-Solute Carrier Family 25 Member 31 antibodyAnti-Sperm Flagellar Energy Carrier Protein antibodyAnti-SLC25A31 antibodyAnti-AAC4 antibodyAnti-ANT4 antibodyAnti-SFEC antibody |
Information sourced from Uniprot.org