| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ADP/ATP translocase 4 (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC25A31 |
| Gene ID: |
83447 |
| Uniprot ID: |
ADT4_HUMAN |
| Immunogen Region: |
131-180 aa |
| Specificity: |
ANT4 Polyclonal Antibody detects endogenous levels of ANT4 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human SLC25A31 at the amino acid range 131-180 |
| Function | ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. Specifically required during spermatogenesis, probably to mediate ADP:ATP exchange in spermatocytes. Large ATP supplies from mitochondria may be critical for normal progression of spermatogenesis during early stages of meiotic prophase I, including DNA double-strand break repair and chromosomal synapsis. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A31/ANT4 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Among nucleotides, may also exchange ADP for dATP and dADP. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A31/ANT4 constitutes a pore-forming component of mPTP or regulates it. |
| Protein Name | Adp/Atp Translocase 4Adp -Atp Carrier Protein 4Adenine Nucleotide Translocator 4Ant 4Solute Carrier Family 25 Member 31Sperm Flagellar Energy Carrier Protein |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane ProteinMembraneCell ProjectionCiliumFlagellum MembraneIn Sperm Flagellum This Protein Is Located In The Fibrous SheathA Non-Mitochondrial RegionMay Localize To Non-Mitochondrial Membranes |
| Alternative Antibody Names | Anti-Adp/Atp Translocase 4 antibodyAnti-Adp -Atp Carrier Protein 4 antibodyAnti-Adenine Nucleotide Translocator 4 antibodyAnti-Ant 4 antibodyAnti-Solute Carrier Family 25 Member 31 antibodyAnti-Sperm Flagellar Energy Carrier Protein antibodyAnti-SLC25A31 antibodyAnti-AAC4 antibodyAnti-ANT4 antibodyAnti-SFEC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
