• Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using SERCA1/ATP2A1 Rabbit monoclonal antibody (STJ11103064, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using SERCA1/ATP2A1 Rabbit monoclonal antibody (STJ11103064, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Western blot analysis of lysates from Mouse skeletal muscle, using SERCA1/ATP2A1 Rabbit monoclonal antibody (STJ11103064) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SERCA1/ATP2A1 antibody (900-1001) [S4MR] (STJ11103064)

SKU:
STJ11103064

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SERCA1/ATP2A1 (900-1001) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP2A1
Gene ID: 487
Uniprot ID: AT2A1_HUMAN
Immunogen Region: 900-1001
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900-1001 of human SERCA1/ATP2A1 (O14983).
Immunogen Sequence: ALSVLVTIEMCNALNSLSEN QSLLRMPPWVNIWLLGSICL SMSLHFLILYVDPLPMIFKL RALDLTQWLMVLKISLPVIG LDEILKFVARNYLEDPEDER RK
Tissue Specificity Skeletal muscle, fast twitch muscle (type II) fibers.
Function Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Protein Name Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 1
Serca1
Sr Ca(2+-Atpase 1
Calcium Pump 1
Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Fast Twitch Skeletal Muscle Isoform
Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase
Database Links Reactome: R-HSA-1912420
Reactome: R-HSA-418359
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Sarcoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 1 antibody
Anti-Serca1 antibody
Anti-Sr Ca(2+-Atpase 1 antibody
Anti-Calcium Pump 1 antibody
Anti-Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Fast Twitch Skeletal Muscle Isoform antibody
Anti-Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase antibody
Anti-ATP2A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance