• Immunofluorescence analysis of U2OS cells using SCYL2 Rabbit polyclonal antibody (STJ115144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SCYL2 Rabbit polyclonal antibody (STJ115144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SCYL2 Rabbit polyclonal antibody (STJ115144) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SCYL2 antibody (1-300) (STJ115144)

SKU:
STJ115144

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCYL2 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCYL2
Gene ID: 55681
Uniprot ID: SCYL2_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3).
Immunogen Sequence: MESMLNKLKSTVTKVTADVT SAVMGNPVTREFDVGRHIAS GGNGLAWKIFNGTKKSTKQE VAVFVFDKKLIDKYQKFEKD QIIDSLKRGVQQLTRLRHPR LLTVQHPLEESRDCLAFCTE PVFASLANVLGNWENLPSPI SPDIKDYKLYDVETKYGLLQ VSEGLSFLHSSVKMVHGNIT PENIILNKSGAWKIMGFDFC VSSTNPSEQEPKFPCKEWDP NLPSLCLPNPEYLAPEYIL
Post Translational Modifications Could autophosphorylate in presence of poly-L-lysine.
Function Component of the AP2-containing clathrin coat that may regulate clathrin-dependent trafficking at plasma membrane, TGN and endosomal system (Probable). A possible serine/threonine-protein kinase toward the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine has not been confirmed. By regulating the expression of excitatory receptors at synapses, plays an essential role in neuronal function and signaling and in brain development.
Protein Name Scy1-Like Protein 2
Coated Vesicle-Associated Kinase Of 104 Kda
Cellular Localisation Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Golgi Apparatus
Trans-Golgi Network Membrane
Endosome Membrane
Colocalizes To The Trans-Golgi Network (Tgn) And To Endosomal Membranes With Clathrin
Transferrin And Plasma Membrane Adapter Ap1 And Ap3 Complexes
Alternative Antibody Names Anti-Scy1-Like Protein 2 antibody
Anti-Coated Vesicle-Associated Kinase Of 104 Kda antibody
Anti-SCYL2 antibody
Anti-CVAK104 antibody
Anti-KIAA1360 antibody

Information sourced from Uniprot.org

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