| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SCYL2 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SCYL2 |
| Gene ID: |
55681 |
| Uniprot ID: |
SCYL2_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3). |
| Immunogen Sequence: |
MESMLNKLKSTVTKVTADVT SAVMGNPVTREFDVGRHIAS GGNGLAWKIFNGTKKSTKQE VAVFVFDKKLIDKYQKFEKD QIIDSLKRGVQQLTRLRHPR LLTVQHPLEESRDCLAFCTE PVFASLANVLGNWENLPSPI SPDIKDYKLYDVETKYGLLQ VSEGLSFLHSSVKMVHGNIT PENIILNKSGAWKIMGFDFC VSSTNPSEQEPKFPCKEWDP NLPSLCLPNPEYLAPEYIL |
| Post Translational Modifications | Could autophosphorylate in presence of poly-L-lysine. |
| Function | Component of the AP2-containing clathrin coat that may regulate clathrin-dependent trafficking at plasma membrane, TGN and endosomal system (Probable). A possible serine/threonine-protein kinase toward the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine has not been confirmed. By regulating the expression of excitatory receptors at synapses, plays an essential role in neuronal function and signaling and in brain development. |
| Protein Name | Scy1-Like Protein 2Coated Vesicle-Associated Kinase Of 104 Kda |
| Cellular Localisation | Cytoplasmic VesicleClathrin-Coated VesicleGolgi ApparatusTrans-Golgi Network MembraneEndosome MembraneColocalizes To The Trans-Golgi Network (Tgn) And To Endosomal Membranes With ClathrinTransferrin And Plasma Membrane Adapter Ap1 And Ap3 Complexes |
| Alternative Antibody Names | Anti-Scy1-Like Protein 2 antibodyAnti-Coated Vesicle-Associated Kinase Of 104 Kda antibodyAnti-SCYL2 antibodyAnti-CVAK104 antibodyAnti-KIAA1360 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
