Anti-SCNN1A antibody (390-440 aa) (STJ13102020)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 390-440 of the extracellular domain of human SCNN1A conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-SCNN1A (390-440 aa) for use in IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | SCNN1A |
| Gene ID | 6337 |
| Uniprot ID | SCNNA_HUMAN |
| Immunogen | A synthetic peptide from aa region 390-440 of the extracellular domain of human SCNN1A conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | 390-440 aa |
| Specificity | Specific for SCNN1A. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1A subunit, which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract. BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC. N-glycosylated. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. Isoform 4: Not functional. |
| Protein Name | Epithelial Sodium Channel Subunit AlphaAlpha-EnacEnac Subunit AlphaEnacaEpithelial Na(+ Channel Subunit AlphaAlpha-NachAmiloride-Sensitive Sodium Channel Subunit AlphaNonvoltage-Gated Sodium Channel 1 Subunit AlphaScneaSodium Channel Epithelial 1 Subunit Alpha |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCytoplasmic GranuleCytoplasmCytoplasmic VesicleSecretory VesicleAcrosomeFlagellumIn The Oviduct And BronchusLocated On Cilia In Multi-Ciliated CellsIn Endometrial Non-Ciliated Epithelial CellsRestricted To Apical SurfacesIn EpidermisLocated Nearly Uniformly In The Cytoplasm In A Granular DistributionIn Sebaceous GlandsObserved Only In The Cytoplasmic Space In Between The Lipid VesiclesIn Eccrine Sweat GlandsMainly Located At The Apical Surface Of The Cells Facing The LumenIn SkinIn Arrector Pili Muscle Cells And In AdipocytesLocated In The Cytoplasm And Colocalized With Actin FibersIn SpermatogoniaSpermatocytes And Round SpermatidsLocated In The CytoplasmPrior To SpermiationLocation Shifts From The Cytoplasm To The Spermatid TailIn SpermatozoaLocalizes At The Acrosome And The Central Region Of The Sperm Flagellum |
| Alternative Antibody Names | Anti-Epithelial Sodium Channel Subunit Alpha antibodyAnti-Alpha-Enac antibodyAnti-Enac Subunit Alpha antibodyAnti-Enaca antibodyAnti-Epithelial Na(+ Channel Subunit Alpha antibodyAnti-Alpha-Nach antibodyAnti-Amiloride-Sensitive Sodium Channel Subunit Alpha antibodyAnti-Nonvoltage-Gated Sodium Channel 1 Subunit Alpha antibodyAnti-Scnea antibodyAnti-Sodium Channel Epithelial 1 Subunit Alpha antibodyAnti-SCNN1A antibodyAnti-SCNN1 antibody |
Information sourced from Uniprot.org