| Host: | Rabbit |
| Applications: | IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-SCNN1A (390-440 aa) is suitable for use in Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | SCNN1A |
| Gene ID: | 6337 |
| Uniprot ID: | SCNNA_HUMAN |
| Immunogen Region: | 390-440 aa |
| Specificity: | Specific for SCNN1A. |
| Immunogen: | A synthetic peptide from aa region 390-440 of the extracellular domain of human SCNN1A conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Ubiquitinated.this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation. N-glycosylated. |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception. |
| Protein Name | Amiloride-Sensitive Sodium Channel Subunit Alpha Alpha-Nach Epithelial Na(+ Channel Subunit Alpha Alpha-Enac Enaca Nonvoltage-Gated Sodium Channel 1 Subunit Alpha Scnea |
| Database Links | Reactome: R-HSA-2672351 Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Cell Projection Cilium Cytoplasmic Granule Cytoplasm Cytoplasmic Vesicle Secretory Vesicle Acrosome Flagellum In The Oviduct And Bronchus Located On Cilia In Multi-Ciliated Cells In Endometrial Non-Ciliated Epithelial Cells Restricted To Apical Surfaces In Epidermis Located Nearly Uniformly In The Cytoplasm In A Granular Distribution In Sebaceous Glands Observed Only In The Cytoplasmic Space In Between The Lipid Vesicles In Eccrine Sweat Glands Mainly Located At The Apical Surface Of The Cells Facing The Lumen In Skin In Arrector Pili Muscle Cells And In Adipocytes Located In The Cytoplasm And Colocalized With Actin Fibers In Spermatogonia Spermatocytes And Round Spermatids Located In The Cytoplasm Prior To Spermiation Location Shifts From The Cytoplasm To The Spermatid Tail In Spermatozoa Localizes At The Acrosome And The Central Region Of The Sperm Flagellum |
| Alternative Antibody Names | Anti-Amiloride-Sensitive Sodium Channel Subunit Alpha antibody Anti-Alpha-Nach antibody Anti-Epithelial Na(+ Channel Subunit Alpha antibody Anti-Alpha-Enac antibody Anti-Enaca antibody Anti-Nonvoltage-Gated Sodium Channel 1 Subunit Alpha antibody Anti-Scnea antibody Anti-SCNN1A antibody Anti-SCNN1 antibody |
Information sourced from Uniprot.org
