• Western blot analysis of extracts of various cell lines, using 67kDa Laminin Receptor antibody (STJ113608) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U-2 OS cells using 67kDa Laminin Receptor Rabbit polyclonal antibody (STJ113608) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using 67kDa Laminin Receptor antibody (STJ113608) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using 67kDa Laminin Receptor Rabbit polyclonal antibody (STJ113608) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using 67kDa Laminin Receptor rabbit polyclonal antibody (STJ113608) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit polyclonal antibody (STJ113608) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U-2 OS cells using 67kDa Laminin Receptor rabbit polyclonal antibody (STJ113608) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit polyclonal antibody (STJ113608) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPSA antibody (1-295) (STJ113608)

SKU:
STJ113608

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Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPSA (1-295) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPSA
Gene ID: 3921
Uniprot ID: RSSA_HUMAN
Immunogen Region: 1-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human 67kDa Laminin Receptor (NP_002286.2).
Immunogen Sequence: MSGALDVLQMKEEDVLKFLA AGTHLGGTNLDFQMEQYIYK RKSDGIYIINLKRTWEKLLL AARAIVAIENPADVSVISSR NTGQRAVLKFAAATGATPIA GRFTPGTFTNQIQAAFREPR LLVVTDPRADHQPLTEASYV NLPTIALCNTDSPLRYVDIA IPCNNKGAHSVGLMWWMLAR EVLRMRGTISREHPWEVMPD LYFYRDPEEIEKEEQAAAEK AVTKEEFQGEWTAPAPEFT
Post Translational Modifications Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association. Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
Function Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9. (Microbial infection) Acts as a receptor for the Dengue virus. (Microbial infection) Acts as a receptor for the Sindbis virus. (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus. (Microbial infection) Acts as a receptor for the pathogenic prion protein. (Microbial infection) Acts as a receptor for bacteria.
Protein Name Small Ribosomal Subunit Protein Us2
37 Kda Laminin Receptor Precursor
37lrp
37/67 Kda Laminin Receptor
Lrp/Lr
40s Ribosomal Protein Sa
67 Kda Laminin Receptor
67lr
Colon Carcinoma Laminin-Binding Protein
Laminin Receptor 1
Lamr
Laminin-Binding Protein Precursor P40
Lbp/P40
Multidrug Resistance-Associated Protein Mgr1-Ag
Nem/1chd4
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cell Membrane
Cytoplasm
Nucleus
67lr Is Found At The Surface Of The Plasma Membrane
With Its C-Terminal Laminin-Binding Domain Accessible To Extracellular Ligands
37lrp Is Found At The Cell Surface
In The Cytoplasm And In The Nucleus
Colocalizes With Ppp1r16b In The Cell Membrane
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Us2 antibody
Anti-37 Kda Laminin Receptor Precursor antibody
Anti-37lrp antibody
Anti-37/67 Kda Laminin Receptor antibody
Anti-Lrp/Lr antibody
Anti-40s Ribosomal Protein Sa antibody
Anti-67 Kda Laminin Receptor antibody
Anti-67lr antibody
Anti-Colon Carcinoma Laminin-Binding Protein antibody
Anti-Laminin Receptor 1 antibody
Anti-Lamr antibody
Anti-Laminin-Binding Protein Precursor P40 antibody
Anti-Lbp/P40 antibody
Anti-Multidrug Resistance-Associated Protein Mgr1-Ag antibody
Anti-Nem/1chd4 antibody
Anti-RPSA antibody
Anti-LAMBR antibody
Anti-LAMR1 antibody

Information sourced from Uniprot.org

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