| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF149 |
| Gene ID: | 284996 |
| Uniprot ID: | RN149_HUMAN |
| Immunogen Region: | 221-400 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2). |
| Immunogen Sequence: | FYYIQRFLYTGSQIGSQSHR KETKKVIGQLLLHTVKHGEK GIDVDAENCAVCIENFKVKD IIRILPCKHIFHRICIDPWL LDHRTCPMCKLDVIKALGYW GEPGDVQEMPAPESPPGRDP AANLSLALPDDDGSDDSSPP SASPAESEPQCDPSFKGDAG ENTALLEAGRSDSRHGGPIS |
| Function | E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf149Dna Polymerase-Transactivated Protein 2Ring Finger Protein 149Ring-Type E3 Ubiquitin Transferase Rnf149 |
| Cellular Localisation | MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf149 antibodyAnti-Dna Polymerase-Transactivated Protein 2 antibodyAnti-Ring Finger Protein 149 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf149 antibodyAnti-RNF149 antibodyAnti-DNAPTP2 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/100398/386820/STJ25258_1__87091.1713155968.jpg?c=1 "Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
