• Western blot analysis of extracts of various cell lines, using (STJ25355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RHOT2 rabbit polyclonal antibody (STJ25355) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RHOT2 rabbit polyclonal antibody (STJ25355) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using RHOT2 rabbit polyclonal antibody (STJ25355) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RHOT2 antibody (340-550) (STJ25355)

SKU:
STJ25355

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHOT2 (340-550) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOT2
Gene ID: 89941
Uniprot ID: MIRO2_HUMAN
Immunogen Region: 340-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-550 of human RHOT2 (NP_620124.1).
Immunogen Sequence: PWGPELPRTVRTEAGRLPLH GYLCQWTLVTYLDVRSCLGH LGYLGYPTLCEQDQAHAITV TREKRLDQEKGQTQRSVLLC KVVGARGVGKSAFLQAFLGR GLGHQDTREQPPGYAIDTVQ VNGQEKYLILCEVGTDGLLA TSLDATCDVACLMFDGSDPK SFAHCASVYKHHYMDGQTPC LFVSSKADLPEGVAVSGPSP AEFCRKHRLPA
Tissue Specificity Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Ubiquitinated by PRKN in a PINK1-dependent manner, leading to its degradation.
Function Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Protein Name Mitochondrial Rho Gtpase 2
Miro-2
Hmiro-2
Ras Homolog Gene Family Member T2
Database Links Reactome: R-HSA-9013419
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Colocalizes With Mgarp And Rhot2 At The Mitochondria
Alternative Antibody Names Anti-Mitochondrial Rho Gtpase 2 antibody
Anti-Miro-2 antibody
Anti-Hmiro-2 antibody
Anti-Ras Homolog Gene Family Member T2 antibody
Anti-RHOT2 antibody
Anti-ARHT2 antibody
Anti-C16orf39 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance