• Anti-RFC4 antibody (204-363) (STJ27438)
  • Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g RFC4 antibody (STJ27438). Western blot was performed from the immunoprecipitate using RFC4 antibody (STJ27438) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using RFC4 Rabbit polyclonal antibody (STJ27438). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RFC4 Rabbit polyclonal antibody (STJ27438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RFC4 antibody (204-363) (STJ27438)

SKU:
STJ27438

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RFC4 (204-363) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFC4
Gene ID: 5984
Uniprot ID: RFC4_HUMAN
Immunogen Region: 204-363
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Immunogen Sequence: DKIQQQRLLDIAKKENVKIS DEGIAYLVKVSEGDLRKAIT FLQSATRLTGGKEITEKVIT DIAGVIPAEKIDGVFAACQS GSFDKLEAVVKDLIDEGHAA TQLVNQLHDVVVENNLSDKQ KSIITEKLAEVDKCLADGAD EHLQLISLCATVMQQLSQNC
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
Protein Name Replication Factor C Subunit 4
Activator 1 37 Kda Subunit
A1 37 Kda Subunit
Activator 1 Subunit 4
Replication Factor C 37 Kda Subunit
Rf-C 37 Kda Subunit
Rfc37
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174411
Reactome: R-HSA-176187
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804756
Reactome: R-HSA-69091
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Replication Factor C Subunit 4 antibody
Anti-Activator 1 37 Kda Subunit antibody
Anti-A1 37 Kda Subunit antibody
Anti-Activator 1 Subunit 4 antibody
Anti-Replication Factor C 37 Kda Subunit antibody
Anti-Rf-C 37 Kda Subunit antibody
Anti-Rfc37 antibody
Anti-RFC4 antibody

Information sourced from Uniprot.org

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