Anti-PRF1 antibody (280-555) (STJ25109)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Perforin (280-555) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRF1 |
| Gene ID | 5551 |
| Uniprot ID | PERF_HUMAN |
| Immunogen Region | 280-555 |
| Immunogen Sequence | EEKKKKHKMTASFHQTYRER HSEVVGGHHTSINDLLFGIQ AGPEQYSAWVNSLPGSPGLV DYTLEPLHVLLDSQDPRREA LRRALSQYLTDRARWRDCSR PCPPGRQKSPRDPCQCVCHG SAVTTQDCCPRQRGLAQLEV TFIQAWGLWGDWFTATDAYV KLFFGGQELRTSTVWDNNNP IWSVRLDFGDVLLATGGPLR LQVWDQDSGRDDDLLGTCDQ APKSGSHEVRCNLNHGHLK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_005032.2). |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1P1CytolysinLymphocyte Pore-Forming ProteinPfp |
| Database Links | Reactome: R-HSA-9725371 |
| Cellular Localisation | Cytolytic GranuleSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenStored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation ChangeMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes |
| Alternative Antibody Names | Anti-Perforin-1 antibodyAnti-P1 antibodyAnti-Cytolysin antibodyAnti-Lymphocyte Pore-Forming Protein antibodyAnti-Pfp antibodyAnti-PRF1 antibodyAnti-PFP antibody |
Information sourced from Uniprot.org