• Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (STJ25109) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Perforin antibody (STJ25109) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Perforin antibody (STJ25109) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Perforin antibody (STJ25109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRF1 antibody (280-555) (STJ25109)

SKU:
STJ25109

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Perforin (280-555) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRF1
Gene ID: 5551
Uniprot ID: PERF_HUMAN
Immunogen Region: 280-555
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_005032.2).
Immunogen Sequence: EEKKKKHKMTASFHQTYRER HSEVVGGHHTSINDLLFGIQ AGPEQYSAWVNSLPGSPGLV DYTLEPLHVLLDSQDPRREA LRRALSQYLTDRARWRDCSR PCPPGRQKSPRDPCQCVCHG SAVTTQDCCPRQRGLAQLEV TFIQAWGLWGDWFTATDAYV KLFFGGQELRTSTVWDNNNP IWSVRLDFGDVLLATGGPLR LQVWDQDSGRDDDLLGTCDQ APKSGSHEVRCNLNHGHLK
Post Translational Modifications N-glycosylated.
Function Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytolytic Granule
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative Antibody Names Anti-Perforin-1 antibody
Anti-P1 antibody
Anti-Cytolysin antibody
Anti-Lymphocyte Pore-Forming Protein antibody
Anti-Pfp antibody
Anti-PRF1 antibody
Anti-PFP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance