• Immunofluorescence analysis of U2OS cells using PLCB1 antibody (STJ25023). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (STJ25023) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (STJ25023) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (STJ25023) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (STJ25023) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PLCB1 antibody (STJ25023)

SKU:
STJ25023

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PLCB1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: PLCB1_HUMAN
Gene ID: 23236
Gene Symbol: PLCB1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
Post Translational Modifications Palmitoylated. Palmitoylation at Cys-17 by ZDHHC21 regulates the signaling activity of PLCB1 and the function of the endothelial barrier. Palmitoylation by ZDHHC21 is stimulated by inflammation.
Function Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors. Regulates the function of the endothelial barrier.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1
Plc-154
Phosphoinositide Phospholipase C-Beta-1
Phospholipase C-I
Plc-I
Phospholipase C-Beta-1
Plc-Beta-1
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-1855204
Reactome: R-HSA-399997
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-418217
Reactome: R-HSA-434316
Reactome: R-HSA-500657
Cellular Localisation Nucleus Membrane
Cytoplasm
Colocalizes With The Adrenergic Receptors
Adren1a And Adren1b
At The Nuclear Membrane Of Cardiac Myocytes
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1 antibody
Anti-Plc-154 antibody
Anti-Phosphoinositide Phospholipase C-Beta-1 antibody
Anti-Phospholipase C-I antibody
Anti-Plc-I antibody
Anti-Phospholipase C-Beta-1 antibody
Anti-Plc-Beta-1 antibody
Anti-PLCB1 antibody
Anti-KIAA0581 antibody

Information sourced from Uniprot.org

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