• Immunohistochemistry analysis of PLCB1 in paraffin-embedded rat brain using PLCB1 Rabbit polyclonal antibody (STJ25023) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells using PLCB1 Rabbit polyclonal antibody (STJ25023) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLCB1 antibody (917-1216) (STJ25023)

SKU:
STJ25023

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLCB1 (917-1216) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCB1
Gene ID: 23236
Uniprot ID: PLCB1_HUMAN
Immunogen Region: 917-1216
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
Immunogen Sequence: KSFVKLQKKHYKEMKDLVKR HHKKTTDLIKEHTTKYNEIQ NDYLRRRAALEKSAKKDSKK KSEPSSPDHGSSTIEQDLAA LDAEMTQKLIDLKDKQQQQL LNLRQEQYYSEKYQKREHIK LLIQKLTDVAEECQNNQLKK LKEICEKEKKELKKKMDKKR QEKITEAKSKDKSQMEEEKT EMIRSYIQEVVQYIKRLEEA QSKRQEKLVEKHKEIRQQIL DEKPKLQVELEQEYQDKFK
Post Translational Modifications Palmitoylated. Palmitoylation at Cys-17 by ZDHHC21 regulates the signaling activity of PLCB1 and the function of the endothelial barrier. Palmitoylation by ZDHHC21 is stimulated by inflammation.
Function Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors. Regulates the function of the endothelial barrier.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1
Plc-154
Phosphoinositide Phospholipase C-Beta-1
Phospholipase C-I
Plc-I
Phospholipase C-Beta-1
Plc-Beta-1
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-1855204
Reactome: R-HSA-399997
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-418217
Reactome: R-HSA-434316
Reactome: R-HSA-500657
Cellular Localisation Nucleus Membrane
Cytoplasm
Colocalizes With The Adrenergic Receptors
Adren1a And Adren1b
At The Nuclear Membrane Of Cardiac Myocytes
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1 antibody
Anti-Plc-154 antibody
Anti-Phosphoinositide Phospholipase C-Beta-1 antibody
Anti-Phospholipase C-I antibody
Anti-Plc-I antibody
Anti-Phospholipase C-Beta-1 antibody
Anti-Plc-Beta-1 antibody
Anti-PLCB1 antibody
Anti-KIAA0581 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance