Anti-PLCB1 antibody (917-1216) (STJ25023)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PLCB1 (917-1216) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLCB1 |
| Gene ID | 23236 |
| Uniprot ID | PLCB1_HUMAN |
| Immunogen Region | 917-1216 |
| Immunogen Sequence | KSFVKLQKKHYKEMKDLVKR HHKKTTDLIKEHTTKYNEIQ NDYLRRRAALEKSAKKDSKK KSEPSSPDHGSSTIEQDLAA LDAEMTQKLIDLKDKQQQQL LNLRQEQYYSEKYQKREHIK LLIQKLTDVAEECQNNQLKK LKEICEKEKKELKKKMDKKR QEKITEAKSKDKSQMEEEKT EMIRSYIQEVVQYIKRLEEA QSKRQEKLVEKHKEIRQQIL DEKPKLQVELEQEYQDKFK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1). |
Additional Info
| Post Translational Modifications | Palmitoylated. Palmitoylation at Cys-17 by ZDHHC21 regulates the signaling activity of PLCB1 and the function of the endothelial barrier. Palmitoylation by ZDHHC21 is stimulated by inflammation. |
| Function | Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors. Regulates the function of the endothelial barrier. |
| Protein Name | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1Plc-154Phosphoinositide Phospholipase C-Beta-1Phospholipase C-IPlc-IPhospholipase C-Beta-1Plc-Beta-1 |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-1855204Reactome: R-HSA-399997Reactome: R-HSA-4086398Reactome: R-HSA-416476Reactome: R-HSA-418217Reactome: R-HSA-434316Reactome: R-HSA-500657 |
| Cellular Localisation | Nucleus MembraneCytoplasmColocalizes With The Adrenergic ReceptorsAdren1a And Adren1bAt The Nuclear Membrane Of Cardiac Myocytes |
| Alternative Antibody Names | Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-1 antibodyAnti-Plc-154 antibodyAnti-Phosphoinositide Phospholipase C-Beta-1 antibodyAnti-Phospholipase C-I antibodyAnti-Plc-I antibodyAnti-Phospholipase C-Beta-1 antibodyAnti-Plc-Beta-1 antibodyAnti-PLCB1 antibodyAnti-KIAA0581 antibody |
Information sourced from Uniprot.org