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Anti-Phospho-SMAD3-Ser204 antibody (170-219 aa) (STJ90523)

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15', using Smad3 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of various cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1：500
Western blot analysis of MCF-7 cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1：500
Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser204) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-SMAD3-Ser204 antibody (170-219 aa) (STJ90523)

SKU:

STJ90523

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Mothers against decapentaplegic homolog 3-Ser204 (170-219 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	SMAD3
Gene ID:	4088
Uniprot ID:	SMAD3_HUMAN
Immunogen Region:	170-219 aa
Specificity:	Phospho-Smad3 (S204) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Smad3 around the phosphorylation site of Ser204 at the amino acid range 170-219

Function	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Protein Name	Mothers Against Decapentaplegic Homolog 3 Mad Homolog 3 Mad3 Mothers Against Dpp Homolog 3 Hmad-3 Jv15-2 Smad Family Member 3 Smad 3 Smad3 Hsmad3
Database Links	Reactome: R-HSA-1181150 Reactome: R-HSA-1502540 Reactome: R-HSA-2173788 Reactome: R-HSA-2173789 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-3304356 Reactome: R-HSA-3311021 Reactome: R-HSA-3315487 Reactome: R-HSA-3656532 Reactome: R-HSA-5689880 Reactome: R-HSA-8941855 Reactome: R-HSA-8952158 Reactome: R-HSA-9008059 Reactome: R-HSA-9013695 Reactome: R-HSA-9615017 Reactome: R-HSA-9617828 Reactome: R-HSA-9735871 Reactome: R-HSA-9754189 Reactome: R-HSA-9796292 Reactome: R-HSA-9823730
Cellular Localisation	Cytoplasm Nucleus Cytoplasmic And Nuclear In The Absence Of Tgf-Beta On Tgf-Beta Stimulation Migrates To The Nucleus When Complexed With Smad4 Through The Action Of The Phosphatase Ppm1a Released From The Smad2/Smad4 Complex And Exported Out Of The Nucleus By Interaction With Ranbp1 Co-Localizes With Lemd3 At The Nucleus Inner Membrane Mapk-Mediated Phosphorylation Appears To Have No Effect On Nuclear Import Pdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm Of The Inner Cell Mass At The Blastocyst Stage
Alternative Antibody Names	Anti-Mothers Against Decapentaplegic Homolog 3 antibody Anti-Mad Homolog 3 antibody Anti-Mad3 antibody Anti-Mothers Against Dpp Homolog 3 antibody Anti-Hmad-3 antibody Anti-Jv15-2 antibody Anti-Smad Family Member 3 antibody Anti-Smad 3 antibody Anti-Smad3 antibody Anti-Hsmad3 antibody Anti-SMAD3 antibody Anti-MADH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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