• Western blot analysis of lysates from HeLa cells treated with UV 15', using RAD17 (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Rad17 (S645) Polyclonal Antibody

Anti-Phospho-RAD17-Ser645 antibody (621-670 aa) (STJ90451)

SKU:
STJ90451

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cell cycle checkpoint protein RAD17-Ser645 (621-670 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD17
Gene ID: 5884
Uniprot ID: RAD17_HUMAN
Immunogen Region: 621-670 aa
Specificity: Phospho-Rad17 (S645) Polyclonal Antibody detects endogenous levels of Rad17 protein only when phosphorylated at S645.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RAD17 around the phosphorylation site of Ser645 at the amino acid range 621-670
Post Translational Modifications Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Protein Name Cell Cycle Checkpoint Protein Rad17
Hrad17
Rf-C/Activator 1 Homolog
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Phosphorylated Form Redistributes To Discrete Nuclear Foci Upon Dna Damage
Alternative Antibody Names Anti-Cell Cycle Checkpoint Protein Rad17 antibody
Anti-Hrad17 antibody
Anti-Rf-C/Activator 1 Homolog antibody
Anti-RAD17 antibody
Anti-R24L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance