• Western blot analysis of extracts of C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 antibody (STJ117912) at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 Rabbit polyclonal antibody (STJ117912) at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PTPRA-Y789 antibody (STJ117912)

SKU:
STJ117912

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PTPRA-Y789 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRA
Gene ID: 5786
Uniprot ID: PTPRA_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y789 of human PTPRA (NP_543030.1).
Immunogen Sequence: SDYAN
Post Translational Modifications Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.
Function Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation. Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase Alpha
Protein-Tyrosine Phosphatase Alpha
R-Ptp-Alpha
Database Links Reactome: R-HSA-375165
Reactome: R-HSA-5673001
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Localizes To Focal Adhesion Sites Following Integrin Engagement
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase Alpha antibody
Anti-Protein-Tyrosine Phosphatase Alpha antibody
Anti-R-Ptp-Alpha antibody
Anti-PTPRA antibody
Anti-PTPA antibody
Anti-PTPRL2 antibody

Information sourced from Uniprot.org

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