• Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+) , but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-KCC2-Ser940 antibody (STJA0003753)

SKU:
STJA0003753-100

£442.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Mouse/Rat/Bovine/Canine/Non-Human Primates/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-KCC2-Ser940 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP 40ul of antibody per 1mg lysate
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Slc12a5
Gene ID: 171373
Uniprot ID: S12A5_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH.
Tissue Specificity Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions.
Post Translational Modifications Phosphorylated at Thr-929 and Thr-1030 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity.
Function Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.
Protein Name Solute Carrier Family 12 Member 5
Electroneutral Potassium-Chloride Cotransporter 2
Furosemide-Sensitive K-Cl Cotransporter
K-Cl Cotransporter 2
Rkcc2
Neuronal K-Cl Cotransporter
Database Links Reactome: R-RNO-426117
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Dendrite
Detected On Dendrites
But Not On Axons Of Spinal Cord Neurons And At Gphn-Positive Inhibitory Synapses
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 5 antibody
Anti-Electroneutral Potassium-Chloride Cotransporter 2 antibody
Anti-Furosemide-Sensitive K-Cl Cotransporter antibody
Anti-K-Cl Cotransporter 2 antibody
Anti-Rkcc2 antibody
Anti-Neuronal K-Cl Cotransporter antibody
Anti-Slc12a5 antibody
Anti-Kcc2 antibody

Information sourced from Uniprot.org

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