| Host: |
Rabbit |
| Applications: |
WB/IP |
| Reactivity: |
Mouse/Rat/Bovine/Canine/Non-Human Primates/Zebrafish |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-KCC2-Ser940 is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: |
WB 1:1000IHCICCIP 40ul of antibody per 1mg lysate |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Slc12a5 |
| Gene ID: |
171373 |
| Uniprot ID: |
S12A5_RAT |
| Immunogen: |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH. |
| Tissue Specificity | Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions. |
| Post Translational Modifications | Phosphorylated at Thr-929 and Thr-1030 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. |
| Function | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation. |
| Protein Name | Solute Carrier Family 12 Member 5Electroneutral Potassium-Chloride Cotransporter 2Furosemide-Sensitive K-Cl CotransporterK-Cl Cotransporter 2Rkcc2Neuronal K-Cl Cotransporter |
| Database Links | Reactome: R-RNO-426117 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendriteDetected On DendritesBut Not On Axons Of Spinal Cord Neurons And At Gphn-Positive Inhibitory Synapses |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 5 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 2 antibodyAnti-Furosemide-Sensitive K-Cl Cotransporter antibodyAnti-K-Cl Cotransporter 2 antibodyAnti-Rkcc2 antibodyAnti-Neuronal K-Cl Cotransporter antibodyAnti-Slc12a5 antibodyAnti-Kcc2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
