• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ11101033) at 1:1000 dilution or Histone H3 antibody (A15741). HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (A15741). HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-Histone H3-S10/T11 antibody (STJ11101033)

SKU:
STJ11101033

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3-S10/T11 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S10 & T11 of human Histone H3 (NP_003520.1).
Immunogen Sequence: KSTGG
Background The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Multiple alternative transcripts encoding different isoforms have been described.

Information sourced from Uniprot.org

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