-
Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
-
Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
-
Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ11101033) at 1:1000 dilution or Histone H3 antibody (A15741). HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
-
Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
-
Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
-
Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
-
Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
-
Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
-
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody (STJ11101033) at dilution of 1:100 (40x lens).
-
Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
-
Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
-
Immunohistochemistry analysis of Phospho-Histone H3-S10/T11 in paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
-
Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
-
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (A15741). HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
-
Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 rabbit polyclonal antibody (STJ11101033) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
-
Western blot analysis of various lysates using Phospho-Histone H3-S10/T11 Rabbit polyclonal antibody (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
