Anti-PFKL antibody (700-780) (STJ117838)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PFKL (700-780) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PFKL |
| Gene ID | 5211 |
| Uniprot ID | PFKAL_HUMAN |
| Immunogen Region | 700-780 |
| Immunogen Sequence | FANAPDSACVIGLKKKAVAF SPVTELKKDTDFEHRMPREQ WWLSLRLMLKMLAQYRISMA AYVSGELEHVTRRTLSMDKG F |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3). |
Additional Info
| Post Translational Modifications | GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. |
| Protein Name | Atp-Dependent 6-Phosphofructokinase - Liver TypeAtp-PfkPfk-L6-Phosphofructokinase Type BPhosphofructo-1-Kinase Isozyme BPfk-BPhosphohexokinase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70171 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent 6-Phosphofructokinase - Liver Type antibodyAnti-Atp-Pfk antibodyAnti-Pfk-L antibodyAnti-6-Phosphofructokinase Type B antibodyAnti-Phosphofructo-1-Kinase Isozyme B antibodyAnti-Pfk-B antibodyAnti-Phosphohexokinase antibodyAnti-PFKL antibody |
Information sourced from Uniprot.org