||WB, IHC, IF
||Human, Mouse, Rat
||Rabbit polyclonal anti-Perforin antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
||FOR RESEARCH USE ONLY (RUO).
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||Store at-20°C. Avoid freeze/thaw cycles.
||WB: 1:500-1:2000IHC: 1:50-1:200IF: 1:50-1:200
||Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
| Post Translational Modifications || N-glycosylated. |
| Function || Pore-forming protein that plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. |
| Protein Name || Perforin-1P1CytolysinLymphocyte Pore-Forming ProteinPfp |
| Cellular Localisation || Cytoplasmic Granule LumenSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenStored In Cytoplasmic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation ChangeMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes |
| Alternative Antibody Names || Anti-Perforin-1 antibodyAnti-P1 antibodyAnti-Cytolysin antibodyAnti-Lymphocyte Pore-Forming Protein antibodyAnti-Pfp antibodyAnti-PRF1 antibodyAnti-PFP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance