• Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (STJ25109) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (STJ25109) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (STJ25109) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Perforin antibody (STJ25109) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-Perforin antibody (STJ25109)

SKU:
STJ25109

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-Perforin antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: PERF_HUMAN
Gene ID: 5551
Gene Symbol: PRF1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
Post Translational Modifications N-glycosylated.
Function Pore-forming protein that plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytoplasmic Granule Lumen
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytoplasmic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative Antibody Names Anti-Perforin-1 antibody
Anti-P1 antibody
Anti-Cytolysin antibody
Anti-Lymphocyte Pore-Forming Protein antibody
Anti-Pfp antibody
Anti-PRF1 antibody
Anti-PFP antibody

Information sourced from Uniprot.org

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