• Western blot analysis of various lysates using PACSIN2 Rabbit polyclonal antibody (STJ11104300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PACSIN2 antibody (240-410) (STJ11104300)

SKU:
STJ11104300

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2 (240-410) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PACSIN2
Gene ID: 11252
Uniprot ID: PACN2_HUMAN
Immunogen Region: 240-410
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PACSIN2 (NP_009160.2).
Immunogen Sequence: RLRFFREVLLEVQKHLDLSN VAGYKAIYHDLEQSIRAADA VEDLRWFRANHGPGMAMNWP QFEEWSADLNRTLSRREKKK ATDGVTLTGINQTGDQSLPS KPSSTLNVPSNPAQSAQSQS SYNPFEDEDDTGSTVSEKDD TKAKNVSSYEKTQSYPTDWS DDESNNPFSST
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated by casein kinase 2 (CK2). Phosphorylation by PKC probably decreases the membrane binding and tubulation capacities of PACSIN2, thereby modulating the lifetime of caveolae.
Function Regulates the morphogenesis and endocytosis of caveolae. Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. (Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer. Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes. Involved in lipid droplet formation, which is important for HCV virion assembly.
Protein Name Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2
Syndapin-2
Syndapin-Ii
Sdpii
Database Links Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Early Endosome
Recycling Endosome Membrane
Cell Projection
Ruffle Membrane
Cell Membrane
Membrane
Caveola
Detected At The Neck Of Flask-Shaped Caveolae
Localization To Tubular Recycling Endosomes Probably Requires Interaction With Micall1 And Ehd1
Alternative Antibody Names Anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2 antibody
Anti-Syndapin-2 antibody
Anti-Syndapin-Ii antibody
Anti-Sdpii antibody
Anti-PACSIN2 antibody

Information sourced from Uniprot.org

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