| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2 (240-410) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PACSIN2 |
| Gene ID: |
11252 |
| Uniprot ID: |
PACN2_HUMAN |
| Immunogen Region: |
240-410 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PACSIN2 (NP_009160.2). |
| Immunogen Sequence: |
RLRFFREVLLEVQKHLDLSN VAGYKAIYHDLEQSIRAADA VEDLRWFRANHGPGMAMNWP QFEEWSADLNRTLSRREKKK ATDGVTLTGINQTGDQSLPS KPSSTLNVPSNPAQSAQSQS SYNPFEDEDDTGSTVSEKDD TKAKNVSSYEKTQSYPTDWS DDESNNPFSST |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated by casein kinase 2 (CK2). Phosphorylation by PKC probably decreases the membrane binding and tubulation capacities of PACSIN2, thereby modulating the lifetime of caveolae. |
| Function | Regulates the morphogenesis and endocytosis of caveolae. Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. (Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer. Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes. Involved in lipid droplet formation, which is important for HCV virion assembly. |
| Protein Name | Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2Syndapin-2Syndapin-IiSdpii |
| Database Links | Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmCytoskeletonCytoplasmic Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideEarly EndosomeRecycling Endosome MembraneCell ProjectionRuffle MembraneCell MembraneMembraneCaveolaDetected At The Neck Of Flask-Shaped CaveolaeLocalization To Tubular Recycling Endosomes Probably Requires Interaction With Micall1 And Ehd1 |
| Alternative Antibody Names | Anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2 antibodyAnti-Syndapin-2 antibodyAnti-Syndapin-Ii antibodyAnti-Sdpii antibodyAnti-PACSIN2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PACSIN2 knockout (KO) HeLa cells, using [KO Validated] PACSIN2 Rabbit polyclonal antibody (STJ11100817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89753/358230/STJ11100817_1__18149.1713122560.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PACSIN2 knockout (KO) HeLa cells, using [KO Validated] PACSIN2 Rabbit polyclonal antibody (STJ11100817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
