• Western blot analysis of lysates from HeLa cells, using OGFOD1 Rabbit polyclonal antibody (STJ118970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-OGFOD1 antibody (1-140) (STJ118970)

SKU:
STJ118970

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OGFOD1 (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OGFOD1
Gene ID: 55239
Uniprot ID: OGFD1_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human OGFOD1 (NP_060703.3).
Immunogen Sequence: MNGKRPAEPGPARVGKKGKK EVMAEFSDAVTEETLKKQVA EAWSRRTPFSHEVIVMDMDP FLHCVIPNFIQSQDFLEGLQ KELMNLDFHEKYNDLYKFQQ SDDLKKRREPHISTLRKILF EDFRSWLSDISKIDLESTID
Function Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.
Protein Name Prolyl 3-Hydroxylase Ogfod1
2-Oxoglutarate And Iron-Dependent Oxygenase Domain-Containing Protein 1
Termination And Polyadenylation 1 Homolog
Us12 Prolyl 3-Hydroxylase
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Mainly Nuclear
A Portion Relocalizes To Cytoplasmic Stress Granules Upon Stress
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Prolyl 3-Hydroxylase Ogfod1 antibody
Anti-2-Oxoglutarate And Iron-Dependent Oxygenase Domain-Containing Protein 1 antibody
Anti-Termination And Polyadenylation 1 Homolog antibody
Anti-Us12 Prolyl 3-Hydroxylase antibody
Anti-OGFOD1 antibody
Anti-KIAA1612 antibody
Anti-TPA1 antibody

Information sourced from Uniprot.org

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