• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g OAT antibody (STJ27991). Western blot was performed from the immunoprecipitate using OAT antibody (STJ27991) at a dilution of 1:1000.
  • Immunohistochemistry analysis of OAT in paraffin-embedded human esophageal cancer using OAT Rabbit polyclonal antibody (STJ27991) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of OAT in paraffin-embedded rat kidney using OAT Rabbit polyclonal antibody (STJ27991) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using OAT Rabbit polyclonal antibody (STJ27991) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : RT4. Exposuretime: 30s.

Anti-OAT antibody (160-439) (STJ27991)

SKU:
STJ27991

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OAT (160-439) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OAT
Gene ID: 4942
Uniprot ID: OAT_HUMAN
Immunogen Region: 160-439
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
Immunogen Sequence: VKGIQKYKAKIVFAAGNFWG RTLSAISSSTDPTSYDGFGP FMPGFDIIPYNDLPALERAL QDPNVAAFMVEPIQGEAGVV VPDPGYLMGVRELCTRHQVL FIADEIQTGLARTGRWLAVD YENVRPDIVLLGKALSGGLY PVSAVLCDDDIMLTIKPGEH GSTYGGNPLGCRVAIAALEV LEEENLAENADKLGIILRNE LMKLPSDVVTAVRGKGLLNA IVIKETKDWDAWKVCLRLR
Function Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
Protein Name Ornithine Aminotransferase - Mitochondrial
Ornithine Delta-Aminotransferase
Ornithine--Oxo-Acid Aminotransferase Cleaved Into - Ornithine Aminotransferase - Hepatic Form - Ornithine Aminotransferase - Renal Form
Database Links Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Ornithine Aminotransferase - Mitochondrial antibody
Anti-Ornithine Delta-Aminotransferase antibody
Anti-Ornithine--Oxo-Acid Aminotransferase Cleaved Into - Ornithine Aminotransferase - Hepatic Form - Ornithine Aminotransferase - Renal Form antibody
Anti-OAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance