Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g OAT antibody (STJ27991). Western blot was performed from the immunoprecipitate using OAT antibody (STJ27991) at a dilution of 1:1000.

Anti-OAT antibody (160-439) (STJ27991)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ27991
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General Information

Short DescriptionRabbit polyclonal OAT (160-439) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolOAT
Gene ID4942
Uniprot IDOAT_HUMAN
Immunogen Region160-439
Immunogen SequenceVKGIQKYKAKIVFAAGNFWG RTLSAISSSTDPTSYDGFGP FMPGFDIIPYNDLPALERAL QDPNVAAFMVEPIQGEAGVV VPDPGYLMGVRELCTRHQVL FIADEIQTGLARTGRWLAVD YENVRPDIVLLGKALSGGLY PVSAVLCDDDIMLTIKPGEH GSTYGGNPLGCRVAIAALEV LEEENLAENADKLGIILRNE LMKLPSDVVTAVRGKGLLNA IVIKETKDWDAWKVCLRLR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).

Additional Info

Function Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
Protein Name Ornithine Aminotransferase - Mitochondrial
Ornithine Delta-Aminotransferase
Ornithine--Oxo-Acid Aminotransferase Cleaved Into - Ornithine Aminotransferase - Hepatic Form - Ornithine Aminotransferase - Renal Form
Database Links Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Ornithine Aminotransferase - Mitochondrial antibody
Anti-Ornithine Delta-Aminotransferase antibody
Anti-Ornithine--Oxo-Acid Aminotransferase Cleaved Into - Ornithine Aminotransferase - Hepatic Form - Ornithine Aminotransferase - Renal Form antibody
Anti-OAT antibody

Information sourced from Uniprot.org

Citations

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