![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98093/381689/STJ119221_1__50370.1713149871.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98093/381690/STJ119221_2__44506.1713149872.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of NSDHL in paraffin-embedded Mouse heart using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98093/381691/STJ119221_3__83270.1713149873.jpg?c=1 "Immunohistochemistry analysis of NSDHL in paraffin-embedded Mouse heart using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of NSDHL in paraffin-embedded Human liver cancer using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98093/381692/STJ119221_4__60999.1713149874.jpg?c=1 "Immunohistochemistry analysis of NSDHL in paraffin-embedded Human liver cancer using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and NSDHL knockout (KO) 293T (KO) cells, using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98093/381693/STJ119221_5__15707.1713149875.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NSDHL knockout (KO) 293T (KO) cells, using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98093/381689/STJ119221_1__50370.1713149871.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-NSDHL antibody (1-240) (STJ119221)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NSDHL (1-240) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NSDHL |
| Gene ID | 50814 |
| Uniprot ID | NSDHL_HUMAN |
| Immunogen Region | 1-240 |
| Immunogen Sequence | MEPAVSEPMRDQVARTHLTE DTPKVNADIEKVNQNQAKRC TVIGGSGFLGQHMVEQLLAR GYAVNVFDIQQGFDNPQVRF FLGDLCSRQDLYPALKGVNT VFHCASPPPSSNNKELFYRV NYIGTKNVIETCKEAGVQKL ILTSSASVIFEGVDIKNGTE DLPYAMKPIDYYTETKILQE RAVLGANDPEKNFLTTAIRP HGIFGPRDPQLVPILIEAAR NGKMKFVIGNGKNLVDFTF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NSDHL (NP_057006.1). |
Additional Info
| Tissue Specificity | Brain, heart, liver, lung, kidney, skin and placenta. |
| Function | Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis. Also plays a role in the regulation of the endocytic trafficking of EGFR. |
| Protein Name | Sterol-4-Alpha-Carboxylate 3-Dehydrogenase - DecarboxylatingProtein H105e3 |
| Database Links | Reactome: R-HSA-191273 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinLipid DropletTrafficking Through The Golgi Is Necessary For Er Membrane Localization |
| Alternative Antibody Names | Anti-Sterol-4-Alpha-Carboxylate 3-Dehydrogenase - Decarboxylating antibodyAnti-Protein H105e3 antibodyAnti-NSDHL antibodyAnti-H105E3 antibody |
Information sourced from Uniprot.org