• Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NSDHL in paraffin-embedded Mouse heart using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NSDHL in paraffin-embedded Human liver cancer using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and NSDHL knockout (KO) 293T (KO) cells, using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NSDHL antibody (1-240) (STJ119221)

SKU:
STJ119221

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSDHL (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSDHL
Gene ID: 50814
Uniprot ID: NSDHL_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NSDHL (NP_057006.1).
Immunogen Sequence: MEPAVSEPMRDQVARTHLTE DTPKVNADIEKVNQNQAKRC TVIGGSGFLGQHMVEQLLAR GYAVNVFDIQQGFDNPQVRF FLGDLCSRQDLYPALKGVNT VFHCASPPPSSNNKELFYRV NYIGTKNVIETCKEAGVQKL ILTSSASVIFEGVDIKNGTE DLPYAMKPIDYYTETKILQE RAVLGANDPEKNFLTTAIRP HGIFGPRDPQLVPILIEAAR NGKMKFVIGNGKNLVDFTF
Tissue Specificity Brain, heart, liver, lung, kidney, skin and placenta.
Function Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis. Also plays a role in the regulation of the endocytic trafficking of EGFR.
Protein Name Sterol-4-Alpha-Carboxylate 3-Dehydrogenase - Decarboxylating
Protein H105e3
Database Links Reactome: R-HSA-191273
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Lipid Droplet
Trafficking Through The Golgi Is Necessary For Er Membrane Localization
Alternative Antibody Names Anti-Sterol-4-Alpha-Carboxylate 3-Dehydrogenase - Decarboxylating antibody
Anti-Protein H105e3 antibody
Anti-NSDHL antibody
Anti-H105E3 antibody

Information sourced from Uniprot.org

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